NGS1
Sequencing technologies
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| Frederick Sanger | Craig Venter |
Exercises
In all exercises please pay attention to the signal that is used to detect the incorporation of the nucleotide in the growing chain. You do not have to spend the time explaining how the fragments are generated and amplified.
- Exercise 1: Sanger sequencing
- Khan academy intro, we are interested only in the part about the Sanger sequencing
- video 1
- video 2
- Exercise 2: ion semiconductor sequencing (IonTorrent)
- Exercise 3: (dye) sequencing by synthesis (Illumina)
- Illumina , especially from 2 min 15 sec on
- on Wikipedia, especially sec 2.5, "Sequencing by synthesis"
- Exercise 4: sequencing by nanopores (Nanoporetech)